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Description
Description of the bug
I tried to run the NanoSeq pipeline on a fastq.gz file using the following commands. Several steps were completed, but the minimap2 step failed.
Command used and terminal output
The commands I used:
nextflow run nf-core/nanoseq -r 3.1.0 \
--input /samplesheet.csv \
--outdir nanoseq \
--protocol cDNA \
--skip_demultiplexing \
-profile singularity \
--jaffal_ref_dir /for_jaffal \
-c hpc_min2.config
The error message:
=================================== Stage get_fasta (DRR095878) ====================================
java -ea -Xms300m -cp /usr/local/opt/bbmap-38.96-1/current/ jgi.ReformatReads threads=16 ignorebadquality=t in=DRR095878.fastq.gz out=DRR095878.fastq/DRR095878.fastq.fasta
Executing jgi.ReformatReads [threads=16, ignorebadquality=t, in=DRR095878.fastq.gz, out=DRR095878.fastq/DRR095878.fastq.fasta]
Set threads to 16
Input is being processed as unpaired
Changed from ASCII-33 to ASCII-64 on input <: 60 -> 29
Input: 640277 reads 3101342227 bases
Output: 640277 reads (100.00%) 3101342227 bases (100.00%)
Time: 459.405 seconds.
Reads Processed: 640k 1.39k reads/sec
Bases Processed: 3101m 6.75m bases/sec
============================= Stage minimap2_transcriptome (DRR095878) =============================
bash: line 1: minimap2: command not found
Cleaned up file DRR095878.fastq/DRR095878.fastq.paf to .bpipe/trash/DRR095878.fastq.paf
ERROR: stage minimap2_transcriptome failed: Command in stage minimap2_transcriptome failed with exit status = 127 :
minimap2 -t 16 -x map-ont -c for_jaffal/hg38_genCode22.fa DRR095878.fastq/DRR095878.fastq.fasta > DRR095878.fastq/DRR095878.fastq.paf ;
========================================= Pipeline Failed ==========================================
In stage Unknown: One or more parallel stages aborted. The following messages were reported:
--------------------------- minimap2_transcriptome ( DRR095878.fastq ) ---------------------------
Command in stage minimap2_transcriptome failed with exit status = 127 :
minimap2 -t 16 -x map-ont -c for_jaffal/hg38_genCode22.fa DRR095878.fastq/DRR095878.fastq.fasta > DRR095878.fastq/DRR095878.fastq.paf ;
----------------------------------------------------------------------------------------------------
Use 'bpipe errors' to see output from failed commands.Relevant files
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Juassis, orenson and usama-khan1998
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bugSomething isn't workingSomething isn't working