@@ -17,91 +17,91 @@ disable_version_detection: true
1717data_format : " yaml"
1818
1919run_modules :
20- - fastqc
21- - fastp
22- - adapterremoval
23- - custom_content
24- - bowtie2
25- - busco
26- - quast
27- - kraken
28- - prokka
29- - porechop
30- - filtlong
20+ - fastqc
21+ - fastp
22+ - adapterremoval
23+ - custom_content
24+ - bowtie2
25+ - busco
26+ - quast
27+ - kraken
28+ - prokka
29+ - porechop
30+ - filtlong
3131
3232# # Module order
3333top_modules :
34- - " fastqc " :
35- name : " FastQC: raw reads"
36- path_filters_exclude :
37- - " *trimmed*"
38- - " fastp"
39- - " adapterremoval"
40- - " porechop"
41- - " filtlong"
42- - " fastqc " :
43- name : " FastQC: after preprocessing"
44- info : " After trimming and, if requested, contamination removal."
45- path_filters :
46- - " *trimmed*"
47- - " bowtie2 " :
48- name : " Bowtie2: PhiX removal"
49- info : " Mapping statistics of reads mapped against PhiX and subsequently removed."
50- path_filters :
51- - " *_phix_removed.bowtie2.log"
52- - " bowtie2 " :
53- name : " Bowtie2: host removal"
54- info : " Mapping statistics of reads mapped against host genome and subsequently
55- removed."
56- path_filters :
57- - " *_host_removed.bowtie2.log"
58- - " kraken " :
59- name : " Kraken2"
60- anchor : " Kraken2"
61- target : " Kraken2"
62- doi : " 10.1101/gr.210641.116"
63- path_filters :
64- - " *.kraken2_report.txt"
65- - " kraken " :
66- name : " Centrifuge"
67- anchor : " centrifuge"
68- target : " Centrifuge"
69- doi : " 10.1101/gr.210641.116"
70- info : " is a very rapid and memory-efficient system for the classification of DNA
71- sequences from microbial samples. The system uses a novel indexing scheme based
72- on the Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index.
73- Note: Figure title"
74- extra : " ℹ️: plot title will say Kraken2 due to Centrifuge producing the same output
75- format as Kraken. If activated, see the actual Kraken2 results in the section
76- above."
77- path_filters :
78- - " *.centrifuge_kreport.txt"
79- - " quast " :
80- name : " QUAST: assembly"
81- info : " Assembly statistics of raw assemblies."
82- path_filters :
83- - " *rawassemblies.tsv"
84- - " bowtie2 " :
85- name : " Bowtie2: assembly"
86- info : " Mapping statistics of reads mapped against assemblies."
87- path_filters_exclude :
88- - " *_host_removed.bowtie2.log"
89- - " *_phix_removed.bowtie2.log"
90- - " bcftools"
91- - custom_content
92- - " quast " :
93- name : " QUAST: bins"
94- info : " Assembly statistics of binned assemblies."
95- path_filters_exclude :
96- - " *rawassemblies.tsv"
97- - " busco " :
98- info : " assesses genome assembly and annotation completeness with Benchmarking
99- Universal Single-Copy Orthologs. In case BUSCO's automated lineage selection
100- was used, only generic results for the selected domain are shown and only for
101- genome bins and kept, unbinned contigs for which the BUSCO analysis was successfull,
102- i.e. not for contigs for which no BUSCO genes could be found. Bins for which
103- a specific virus lineage was selected are also not shown."
104- - " prokka"
34+ - " fastqc " :
35+ name : " FastQC: raw reads"
36+ path_filters_exclude :
37+ - " *trimmed*"
38+ - " fastp"
39+ - " adapterremoval"
40+ - " porechop"
41+ - " filtlong"
42+ - " fastqc " :
43+ name : " FastQC: after preprocessing"
44+ info : " After trimming and, if requested, contamination removal."
45+ path_filters :
46+ - " *trimmed*"
47+ - " bowtie2 " :
48+ name : " Bowtie2: PhiX removal"
49+ info : " Mapping statistics of reads mapped against PhiX and subsequently removed."
50+ path_filters :
51+ - " *_phix_removed.bowtie2.log"
52+ - " bowtie2 " :
53+ name : " Bowtie2: host removal"
54+ info : " Mapping statistics of reads mapped against host genome and subsequently
55+ removed."
56+ path_filters :
57+ - " *_host_removed.bowtie2.log"
58+ - " kraken " :
59+ name : " Kraken2"
60+ anchor : " Kraken2"
61+ target : " Kraken2"
62+ doi : " 10.1101/gr.210641.116"
63+ path_filters :
64+ - " *.kraken2_report.txt"
65+ - " kraken " :
66+ name : " Centrifuge"
67+ anchor : " centrifuge"
68+ target : " Centrifuge"
69+ doi : " 10.1101/gr.210641.116"
70+ info : " is a very rapid and memory-efficient system for the classification of DNA
71+ sequences from microbial samples. The system uses a novel indexing scheme based
72+ on the Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index.
73+ Note: Figure title"
74+ extra : " ℹ️: plot title will say Kraken2 due to Centrifuge producing the same output
75+ format as Kraken. If activated, see the actual Kraken2 results in the section
76+ above."
77+ path_filters :
78+ - " *.centrifuge_kreport.txt"
79+ - " quast " :
80+ name : " QUAST: assembly"
81+ info : " Assembly statistics of raw assemblies."
82+ path_filters :
83+ - " *rawassemblies.tsv"
84+ - " bowtie2 " :
85+ name : " Bowtie2: assembly"
86+ info : " Mapping statistics of reads mapped against assemblies."
87+ path_filters_exclude :
88+ - " *_host_removed.bowtie2.log"
89+ - " *_phix_removed.bowtie2.log"
90+ - " bcftools"
91+ - custom_content
92+ - " quast " :
93+ name : " QUAST: bins"
94+ info : " Assembly statistics of binned assemblies."
95+ path_filters_exclude :
96+ - " *rawassemblies.tsv"
97+ - " busco " :
98+ info : " assesses genome assembly and annotation completeness with Benchmarking
99+ Universal Single-Copy Orthologs. In case BUSCO's automated lineage selection
100+ was used, only generic results for the selected domain are shown and only for
101+ genome bins and kept, unbinned contigs for which the BUSCO analysis was successfull,
102+ i.e. not for contigs for which no BUSCO genes could be found. Bins for which
103+ a specific virus lineage was selected are also not shown."
104+ - " prokka"
105105
106106custom_data :
107107 host_removal :
@@ -131,12 +131,12 @@ sp:
131131
132132# # File name cleaning
133133extra_fn_clean_exts :
134- - " .bowtie2"
135- - " _ar2"
136- - " host_removed"
137- - " phix_removed"
138- - " centrifuge_kreport"
139- - " _fastp"
134+ - " .bowtie2"
135+ - " _ar2"
136+ - " host_removed"
137+ - " phix_removed"
138+ - " centrifuge_kreport"
139+ - " _fastp"
140140
141141# # Prettification
142142custom_logo : " mag_logo_mascot_light.png"
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